Электронный каталог

Статья

Tudor, M.
Enhancing Proton Radiosensitivity of Chondrosarcoma Using Nanoparticle-Based Drug Delivery Approaches: A Comparative Study of High- and Low-Energy Protons / M.Tudor, A.Rzyanina, G.Mytsin, [a.o.] // International Journal of Molecular Sciences [Electronic resource]. – 2024. – Vol. 25, No. 21. – P. 11481. – URL: https://doi.org/10.3390/ijms252111481. – Bibliogr.: 47.

To overcome chondrosarcoma’s (CHS) high chemo- and radioresistance, we used polyethylene glycol-encapsulated iron oxide nanoparticles (IONPs) for the controlled delivery of the chemotherapeutic doxorubicin (IONP&sub(DOX)) to amplify the cytotoxicity of proton radiation therapy. Human 2D CHS SW1353 cells were treated with protons (linear energy transfer (LET): 1.6 and 12.6 keV/µm) with and without IONP&sub(DOX). Cell survival was assayed using a clonogenic test, and genotoxicity was tested through the formation of micronuclei (MN) and γH2AX foci, respectively. Morphology together with spectral fingerprints of nuclei were measured using enhanced dark-field microscopy (EDFM) assembled with a hyperspectral imaging (HI) module and an axial scanning fluorescence module, as well as scanning electron microscopy (SEM) coupled with energy-dispersive X-Ray spectroscopy (EDX). Cell survival was also determined in 3D SW3153 spheroids following treatment with low-LET protons with/without the IONP&sub(DOX) compound. IONP&sub(DOX) increased radiosensitivity following proton irradiation at both LETs in correlation with DNA damage expressed as MN or γH2AX. The IONP&sub(DOX)–low-LET proton combination caused a more lethal effect compared to IONP&sub(DOX)–high-LET protons. CHS cell biological alterations were reflected by the modifications in the hyperspectral images and spectral profiles, emphasizing new possible spectroscopic markers of cancer therapy effects. Our findings show that the proposed treatment combination has the potential to improve the management of CHS.



ОИЯИ = ОИЯИ (JINR)2024
Спец.(статьи,препринты) = С 349 д - Биологическое действие излучений$
Бюллетени = 49/024