Электронный каталог

Статья

Kurakin, S.
Calcium Ions Do Not Influence the A*b(25–35) Triggered Morphological Changes of Lipid Membranes / S.Kurakin, O.Ivankov, E.Dushanov, T.Murugova, E.Ermakova, A.Kuklin, N.Kucerka, [et al.] // Biophysical Chemistry [Electronic resource]. – 2024. – Vol. 313. – P. 107292. – URL: https://doi.org/10.1016/j.bpc.2024.107292. – Bibliogr.: 85.

We have studied the effect of calcium ions (Ca&sup(2+)) at various concentrations on the structure of lipid vesicles in the presence of amyloid-beta peptide A*b(25–35). In particular, we have investigated the influence of calcium ions on the formation of recently documented bicelle-like structures (BLSs) emerged as a result of A*b(25–35) triggered membrane disintegration. First, we have shown by using small-angle X-ray and neutron scattering that peptide molecules rigidify the lipid bilayer of gel phase DPPC unilamellar vesicles (ULVs), while addition of the calcium ions to the system hinders this effect of A*b(25–35). Secondly, the Aβ(25–35) demonstrates a critical peptide concentration at which the BLSs reorganize from ULVs due to heating and cooling the samples through the lipid main phase transition temperature (T&sub(m)). However, addition of calcium ions does not affect noticeably the A*b-induced formation of BLSs and their structural parameters, though the changes in peptide's secondary structure, e.g. the increased *a-helix fraction, has been registered by circular dichroism spectroscopy. Finally, according to *3*1P nuclear magnetic resonance (NMR) measurements, calcium ions do not affect the lipid-peptide arrangement in BLSs and their ability to align in the magnetic field of NMR spectrometer. The influences of various concentrations of calcium ions on the lipid-peptide interactions may prove biologically important because their local concentrations vary widely in in-vivo conditions. In the present work, calcium ions were investigated as a possible tool aimed at regulating the lipid-peptide interactions that demonstrated the disruptive effect of A*b(25–35) on lipid membranes.



Спец.(статьи,препринты) = С 342 г1 - Замедление и диффузия нейтронов. Дифракция
Спец.(статьи,препринты) = С 44 б - Разделение химических элементов экстракционными и ионообменными методами
Спец.(статьи,препринты) = С 332.8 - Синхротронное излучение. Лазеры на свободных электронах. Получение и использование рентгеновских лучей
ОИЯИ = ОИЯИ (JINR)2024
Бюллетени = 44/024