Электронный каталог

Статья

Belousov, A.
Monitoring GPCR Conformation with GFP-Inspired Dyes / A.Belousov, V.Borshchevskiy, [et al.] // iScience [Electronic resource]. – 2024. – Vol. 27, No. 8. – P. 110466. – URL: https://doi.org/10.1016/j.isci.2024.110466. – Bibliogr.: 107.

Solvatochromic compounds have emerged as valuable environment-sensitive probes for biological research. Here we used thiol-reactive solvatochromic analogs of the green fluorescent protein (GFP) chromophore to track conformational changes in two proteins, recoverin and the A&sub(2A) adenosine receptor (A&sub(2A)AR). Two dyes showed Ca&sup(2+)-induced fluorescence changes when attached to recoverin. Our best-performing dye, DyeC, exhibited agonist-induced changes in both intensity and shape of its fluorescence spectrum when attached to A&sub(2A)AR; none of these effects were observed with other common environment-sensitive dyes. Molecular dynamics simulations showed that activation of the A&sub(2A)AR led to a more confined and hydrophilic environment for DyeC. Additionally, an allosteric modulator of A&sub(2A)AR induced distinct fluorescence changes in the DyeC spectrum, indicating a unique receptor conformation. Our study demonstrated that GFP-inspired dyes are effective for detecting structural changes in G protein-coupled receptors (GPCRs), offering advantages such as intensity-based and ratiometric tracking, redshifted fluorescence spectra, and sensitivity to allosteric modulation.



Спец.(статьи,препринты) = С 44 - Аналитическая химия
Спец.(статьи,препринты) = 28.0 - Биология$
ОИЯИ = ОИЯИ (JINR)2024
Бюллетени = 43/024