Электронный каталог

Статья

Zarubin, M.
The Tardigrade Dsup Protein Enhances Radioresistance in Drosophila Melanogaster and Acts as an Unspecific Repressor of Transcription / M.Zarubin, T.Azorskaya, O.Kuldoshina, S.Alekseev, S.Mitrofanov, E.Kravchenko // iScience [Electronic resource]. – 2023. – Vol.26, No.7. – P.106998. – URL: https://doi.org/10.1016/j.isci.2023.106998. – Bibliogr.:52.

The tardigrade-unique damage suppressor protein (Dsup) can protect DNA from ionizing radiation and reactive oxygen species (ROS). In this study, we generated Dsup-expressing lines of Drosophila melanogaster and demonstrated that Dsup increased the survival rate after γ-ray irradiation and hydrogen peroxide treatment in flies too, but reduced the level of their locomotor activity. The transcriptome analyses of Dsup-expressing lines revealed a significant number of DEGs, >99% of which were down-regulated. Moreover, Dsup could bind RNA. These findings suggest that Dsup can act not only as a DNA protector but also as a non-specific transcriptional repressor and RNA-binding protein, that may lead to disturbance of a number of biological processes in D. melanogaster. The obtained data demonstrate features of the Dsup protein action in non-tardigrade organisms and can be used to understand the impact of other unspecific DNA/RNA-binding proteins on ROS and radiation resistance, gene expression, and epigenetic processes.



ОИЯИ = ОИЯИ (JINR)2023
Спец.(статьи,препринты) = С 349 д - Биологическое действие излучений$
Бюллетени = 4/024